SRRM2 Rabbit pAb

Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SRRM2 Polyclonal Antibody, Unconjugated(AP58857) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF
Primary Accession	Q9UQ35
Reactivity	Mouse
Predicted	Rat, Pig, Dog, Human, Rabbit
Host	Rabbit
Clonality	Polyclonal
Calculated MW	299615 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human SRRM2
Epitope Specificity	101-230/2752
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Nucleus speckle.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	The SRm160/300 splicing coactivator, which consists of the serine/arginine (SR)-related nuclear matrix protein and a nuclear matrix antigen, functions in splicing by promoting critical interactions between splicing factors bound to pre-mRNA. This splicing pathway involves five core small nuclear ribonucleoprotein particles (snRNPs) and the SR family proteins, which coordinately bind to pre-mRNA slicing enhancer elements, are required for accurate splice site recognition, and regulate alterative splicing patterns. The recognized splicing enhancer elements, known also as exonic enhancer splicing sequences, are short RNA sequences that are capable of activating weak splice sites in adjacent introns and contain specific binding sites for the serine/arginine (SR)-rich splicing factors. SRm160 and 300 antigens contain domains rich in SR motifs, but are distinctly different from the SR factors as they lack an RNA recognition motif and cannot directly induce RNA splicing. These proteins rather function as coactivators that stabilize the splicing complex and mediate the U1 snRNP-splicing pathway.

Additional Information
Gene ID	23524
Other Names	Serine/arginine repetitive matrix protein 2, 300 kDa nuclear matrix antigen, Serine/arginine-rich splicing factor-related nuclear matrix protein of 300 kDa, SR-related nuclear matrix protein of 300 kDa, Ser/Arg-related nuclear matrix protein of 300 kDa, Splicing coactivator subunit SRm300, Tax-responsive enhancer element-binding protein 803, TaxREB803, SRRM2, KIAA0324, SRL300, SRM300
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SRRM2
Synonyms	KIAA0324, SRL300, SRM300
Function	Required for pre-mRNA splicing as component of the spliceosome. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).
Cellular Location	Nucleus. Nucleus speckle
Tissue Location	Expressed in liver, placenta, and white blood cells.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol