CCDC136 Rabbit pAb

Tissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CCDC136 Polyclonal Antibody, Unconjugated(AP58866) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF
Primary Accession	Q96JN2
Reactivity	Rat
Predicted	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	134045 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from Human CCDC136/NAG6
Epitope Specificity	85-190/1154
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Membrane; Single-pass membrane protein (Potential).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Expressed in gastric tissues. Down-regulated in gastric cancer.

Additional Information
Gene ID	64753
Other Names	Coiled-coil domain-containing protein 136, Nasopharyngeal carcinoma-associated gene 6 protein, CCDC136, KIAA1793
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CCDC136
Synonyms	KIAA1793
Function	May play a role in acrosome formation in spermatogenesis and in fertilization.
Cellular Location	Cytoplasmic vesicle, secretory vesicle, acrosome membrane {ECO:0000250\|UniProtKB:Q3TVA9}; Single-pass membrane protein {ECO:0000250\|UniProtKB:Q3TVA9}. Note=Exclusively localized on a peripheral part of acrosome membrane (Golgi phase), localized on the equatorial segment of the acrosome (maturation phase) {ECO:0000250\|UniProtKB:Q3TVA9}
Tissue Location	Expressed in gastric tissues. Down-regulated in gastric cancer.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.