CCDC30 Rabbit pAb

Sample:
HepG2 Cell (Human) Lysate at 40 ug
U251 Cell (Human) Lysate at 40 ug
Primary: Anti-CCDC30 (AP58870) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91 kD
Observed band size: 95 kD
Paraformaldehyde-fixed, paraffin embedded (Human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCDC30) Polyclonal Antibody, Unconjugated (AP58870) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q5VVM6
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	91333 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human CCDC30
Epitope Specificity	601-700/783
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Expressed in brain, kidney, pancreas, placenta, liver, thymus and prostate.

Additional Information
Gene ID	728621
Other Names	Coiled-coil domain-containing protein 30, Prefoldin subunit 6-like protein, CCDC30, PFDN6L
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.