CT112 Rabbit pAb

Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cancer/testis antigen 112; CT112) Polyclonal Antibody, Unconjugated (AP58960) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF
Primary Accession	Q8NEK8
Reactivity	Rat, Human
Predicted	Pig, Dog, Mouse, Horse, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	44500 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human FAM46D/CT112
Epitope Specificity	51-150/389
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Antibodies against the protein encoded by this gene were found only in plasma from cancer patients. While it may be a target for immunotherapy, the function of this gene is unknown.

Additional Information
Gene ID	169966
Other Names	Terminal nucleotidyltransferase 5D, 2.7.7.19, Non-canonical poly(A) polymerase FAM46D, TENT5D (HGNC:28399)
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TENT5D (HGNC:28399)
Function	Catalyzes the transfer of one adenosine molecule from an ATP to an mRNA poly(A) tail bearing a 3'-OH terminal group.
Tissue Location	restricted to testis.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.