GK5 Rabbit pAb

Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Lung tissue lysates
Primary: Anti-GK5 (AP58994) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 53 kDa
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GK5) Polyclonal Antibody, Unconjugated (AP58994) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q6ZS86
Reactivity	Rat, Mouse
Predicted	Dog, Human, Rabbit, Horse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	59156 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human GK5/Glycerokinase 5
Epitope Specificity	441-529/529
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	No data available.

Additional Information
Gene ID	256356
Other Names	Glycerol kinase 5, GK 5, Glycerokinase 5, 2.7.1.30, ATP:glycerol 3-phosphotransferase 5, GK5 (HGNC:28635)
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GK5 (HGNC:28635)
Function	Skin-specific kinase that plays a key role in glycerol metabolism, catalyzing its phosphorylation to produce sn-glycerol 3- phosphate. Involved in skin-specific regulation of sterol regulatory element-binding protein (SREBP) processing and lipid biosynthesis.
Cellular Location	Cytoplasm {ECO:0000250\|UniProtKB:Q8BX05}.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.