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GK5 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - GK5 Polyclonal Antibody AP58994
    Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GK5) Polyclonal Antibody, Unconjugated (AP58994) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IHC-F, IF, E
Primary Accession Q6ZS86
Reactivity Rat, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 59156 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human GK5/Glycerokinase 5
Epitope Specificity 441-529/529
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SIMILARITY Belongs to the FGGY kinase family.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions No data available.
Additional Information
Gene ID 256356
Other Names Putative glycerol kinase 5, GK 5, Glycerokinase 5, 2.7.1.30, ATP:glycerol 3-phosphotransferase 5, GK5
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000
Format0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name GK5 (HGNC:28635)
Function Skin-specific kinase that plays a key role in glycerol metabolism, catalyzing its phosphorylation to produce sn-glycerol 3- phosphate. Involved in skin-specific regulation of sterol regulatory element-binding protein (SREBP) processing and lipid biosynthesis.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q8BX05}.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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