FBXL3 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q9UKT7
Reactivity	Rat, Pig, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	48707 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human FBXL3
Epitope Specificity	151-250/428
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Nucleus. Cytoplasm. Predominantly nuclear.
SIMILARITY	Contains 1 F-box domain. Contains 7 LRR (leucine-rich) repeats.
SUBUNIT	Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with CRY1 and CRY2.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Substrate-recognition component of some SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex involved in circadian clock function. The SCF(FBXL3) complex acts by mediating ubiquitination and subsequent degradation of CRY1 and CRY2. Recruiter of target protein that may recognize and bind to some phosphorylated proteins and promotes their ubiquitination and degradation.

Additional Information
Gene ID	26224
Other Names	F-box/LRR-repeat protein 3, F-box and leucine-rich repeat protein 3A, F-box/LRR-repeat protein 3A, FBXL3, FBL3A, FBXL3A
Target/Specificity	Widely expressed.
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	FBXL3
Synonyms	FBL3A, FBXL3A
Function	Substrate-recognition component of the SCF(FBXL3) E3 ubiquitin ligase complex involved in circadian rhythm function. Plays a key role in the maintenance of both the speed and the robustness of the circadian clock oscillation (PubMed:17463251, PubMed:23452855, PubMed:27565346). The SCF(FBXL3) complex mainly acts in the nucleus and mediates ubiquitination and subsequent degradation of CRY1 and CRY2 (PubMed:17463251, PubMed:23452855, PubMed:27565346). Activity of the SCF(FBXL3) complex is counteracted by the SCF(FBXL21) complex (PubMed:23452855).
Cellular Location	Nucleus. Cytoplasm. Note=Predominantly nuclear
Tissue Location	Widely expressed..