Pancreatic hormone Rabbit pAb

Paraformaldehyde-fixed, paraffin embedded (Human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pancreatic hormone) Polyclonal Antibody, Unconjugated (AP59031) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF
Primary Accession	P01298
Reactivity	Human
Predicted	Rat, Pig, Dog, Mouse, Rabbit, Horse, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	10445 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human Pancreatic hormone
Epitope Specificity	30-65/95
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Secreted.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma levels of this hormone have been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.

Additional Information
Gene ID	5539
Other Names	Pancreatic polypeptide prohormone, PH, Pancreatic polypeptide Y {ECO:0000312\|HGNC:HGNC:9327}, Obinepitide, Pancreatic polypeptide, HPP, PP, Pancreatic icosapeptide, PI, PPY (HGNC:9327), PNP
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	PPY (HGNC:9327)
Synonyms	PNP
Function	[Pancreatic polypeptide]: Hormone secreted by pancreatic cells that acts as a regulator of pancreatic and gastrointestinal functions probably by signaling through the G protein-coupled receptor NPY4R2.
Cellular Location	Secreted

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.