癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
RNF98 Rabbit pAb
RNF98 Rabbit pAb
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- 实验流程
- 背景知识
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q9NQ86 |
| Predicted | Rat, Pig, Human, Mouse, Rabbit, Horse, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 83013 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human TRIM36/RNF98 |
| Epitope Specificity | 201-300/728 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Cytoplasm (By similarity). Cytoplasmic vesicle, secretory vesicle, acrosome (By similarity). Cytoplasm, cytoskeleton (By similarity). Note=Found in the acrosomal region of elongated spermatids and mature sperm (By similarity). |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | TRIM36 (tripartite motif-containing 36), also known as RNF98 (RING finger protein 98), HAPRIN (haploid germ cell-specific RBCC protein) or RBCC728, is a 728 amino acid protein that belongs to the TRIM/RBCC (Ring finger, B box, coiled-coil) family. Predominantly expressed in prostate, testis and brain with weak expression in heart, kidney and lung, TRIM36 contains two B box-type zinc fingers, a SPRY domain, a coiled-coil domain, a fibronectin type-III domain and a RING-type zinc finger; a motif that has zinc-chelating activity and is involved in mediating protein-protein and protein-DNA interactions. Localizing to the cytoplasm and the acrosomal region of germ cells and mature sperm, TRIM36 is believed to play a role in the acrosome reaction and fertilization. In addition, TRIM36 is overexpressed in prostate cancer, suggesting a possible role for TRIM36 in prostate tumorigenesis. |
| Gene ID | 55521 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase TRIM36, 2.3.2.27, Tripartite motif-containing protein 36, Zinc-binding protein Rbcc728, TRIM36, RBCC728, RNF98 |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | TRIM36 |
|---|---|
| Synonyms | RBCC728, RNF98 |
| Function | E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins including CTNNB1, FOXA2 and AKT1 (PubMed:35768649, PubMed:37875418, PubMed:41323265). Involved in chromosome segregation and cell cycle regulation (PubMed:28087737). May play a role in the acrosome reaction and fertilization. Inhibits lung fibroblast proliferation, migration, and differentiation. Mechanistically, mediates 'Lys-48'-linked polyubiquitination of phospho-AKT1, leading to its proteasomal degradation and consequent inhibition of AKT signaling (PubMed:41323265). Promotes apoptosis via inhibition of the Wnt/beta- catenin signaling pathway (PubMed:35768649). Regulates ferroptosis by ubiquitinating and degrading FOXA2 (PubMed:37875418). |
| Cellular Location | Cytoplasm. Cytoplasmic vesicle, secretory vesicle, acrosome {ECO:0000250|UniProtKB:Q80WG7}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q80WG7}. Note=Found in the acrosomal region of elongated spermatids and mature sperm. {ECO:0000250|UniProtKB:Q80WG7} |
| Tissue Location | Highly expressed in testis, prostate and brain (PubMed:15145053). Weakly expressed in kidney, lung and heart (PubMed:15145053). Expressed in fetal tissues (PubMed:28087737) |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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