RNF150 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-RNF150 (AP59198) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD
Sample: Lovo Cell (Human) Lysate at 40 ug
Primary: Anti-RNF150 (AP59198) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, ICC, E
Primary Accession	Q9ULK6
Reactivity	Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	48072 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human RNF150
Epitope Specificity	51-150/347
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Contains 1 PA (protease associated) domain.Contains 1 RING-type zinc finger.
SIMILARITY	Contains 1 PA (protease associated) domain.Contains 1 RING-type zinc finger.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in ubiquitin-dependent protein catabolic process. Predicted to be integral component of membrane. Predicted to be active in cytoplasm. [provided by Alliance of Genome Resources, Apr 2022]

Additional Information
Gene ID	57484
Other Names	RING finger protein 150, RNF150, KIAA1214
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:50-200,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.