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BEND5 Rabbit pAb

BEND5 Rabbit pAb

     
  • 0 - BEND5 Rabbit pAb AP59208
    Blank control:Mouse spleen.
    Primary Antibody (green line): Rabbit Anti-BEND5 antibody (AP59208)
    Dilution: 2 µg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-AF488
    Dilution: 1 µg /test.
    Protocol
    The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
  • 4 - BEND5 Rabbit pAb AP59208
    Blank control:Mouse spleen.
    Primary Antibody (green line): Rabbit Anti-BEND5 antibody (AP59208)
    Dilution: 2 µg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-AF488
    Dilution: 1 µg /test.
    Protocol
    The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Product Information
Primary Accession Q7L4P6
Reactivity Mouse
Predicted Rat, Human, Rabbit, Chicken
Host Rabbit
Clonality Polyclonal
Calculated MW 48182 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human BEND5
Epitope Specificity 31-130/421
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Additional Information
Gene ID 79656
Other Names BEN domain-containing protein 5, BEND5, C1orf165
Dilution Flow-Cyt=2ug/Test
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name BEND5
Synonyms C1orf165
Function Acts as a transcriptional repressor (PubMed:23468431).
Research Areas
Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol
Blocking Peptides Protocol
Dot Blot Protocol
Immunohistochemistry Protocol
Immunofluorescence Protocol
Immunoprecipitation Protocol
Flow Cytomety Protocol
Cell Culture Protocol

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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¥ 1,250.00
Cat# AP59208
Size:
50 µl
100 µl
Price:
¥ 1,250.00
¥ 2,050.00
Quantity:
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Availability: 3-5 days
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AP59208: BEND5 Rabbit pAb
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