癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Transcription factor 25 Rabbit pAb
Transcription factor 25 Rabbit pAb
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- 背景知识
Application 
| WB, IHC-P, IHC-F, IF, E |
|---|---|
| Primary Accession | Q9BQ70 |
| Predicted | Human, Mouse, Rat, Pig, Horse, Rabbit |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 76667 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human Transcription factor 25/Nulp1 |
| Epitope Specificity | 201-300/676 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Nuclear. Note=Some staining in the cytosol. |
| SIMILARITY | Belongs to the TCF25 family. |
| SUBUNIT | Interacts with XIAP (By similarity). |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | Transcription factor 25 acts as a transcriptional repressor. It has been shown to repress transcription of SRF in vitro and hence may play a role in heart development.Transcription factor 25, is a 676 amino acid protein that plays a role in cell death. A member of the TCF25 family, Nulp1 utilizes its C-terminus to mediate transcriptional repression of SRF in vitro, and interacts with XIAP. Nulp1 localizes primarily to the nucleus but is also found in cytosol. Widely expressed, Nulp1 is found at high levels in embryonic brain and adult heart. The gene encoding Nulp1 maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is also associated with chromosome 16, as is Crohn's disease, which is a gastrointestinal inflammatory condition. |
| Gene ID | 22980 |
|---|---|
| Other Names | Ribosome quality control complex subunit TCF25, Nuclear localized protein 1, Transcription factor 25, TCF-25, TCF25 {ECO:0000303|PubMed:30244831, ECO:0000312|HGNC:HGNC:29181} |
| Target/Specificity | In the embryo, widely expressed with highest levels in brain. In the adult, highest expression is found in the heart. |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| Name | TCF25 {ECO:0000303|PubMed:30244831, ECO:0000312|HGNC:HGNC:29181} |
|---|---|
| Function | Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation (PubMed:30244831). In the RQC complex, required to promote formation of 'Lys-48'-linked polyubiquitin chains during ubiquitination of incompletely synthesized proteins by LTN1 (PubMed:30244831). May negatively regulate the calcineurin-NFAT signaling cascade by suppressing the activity of transcription factor NFATC4 (By similarity). May play a role in cell death control (By similarity). |
| Cellular Location | Nucleus. Cytoplasm, cytosol. Note=Mainly nuclear |
| Tissue Location | In the embryo, widely expressed with highest levels in brain (PubMed:16574069). In the adult, highest expression is found in the heart (PubMed:16574069, PubMed:32805187). Repressed in cardiac tissue of patients with heart failure (at protein level) (PubMed:32805187). mRNA levels in the heart are unchanged in patients with heart failure (PubMed:32805187). |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Transcription factor 25 acts as a transcriptional repressor. It has been shown to repress transcription of SRF in vitro and hence may play a role in heart development.Transcription factor 25, is a 676 amino acid protein that plays a role in cell death. A member of the TCF25 family, Nulp1 utilizes its C-terminus to mediate transcriptional repression of SRF in vitro, and interacts with XIAP. Nulp1 localizes primarily to the nucleus but is also found in cytosol. Widely expressed, Nulp1 is found at high levels in embryonic brain and adult heart. The gene encoding Nulp1 maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is also associated with chromosome 16, as is Crohn's disease, which is a gastrointestinal inflammatory condition.
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