Anti-BMP3 Antibody

Rabbit polyclonal antibody to BMP3

Western blot analysis of BMP3 expression in A549 (A), H446 (B), U2OS (C), mouse lung (D) whole cell lysates.
Immunohistochemical analysis of BMP3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P12645
Other Accession	Q8BHE5
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	53372 Da

Additional Information
Gene ID	651
Other Names	BMP3A; Bone morphogenetic protein 3; BMP-3; Bone morphogenetic protein 3A; BMP-3A; Osteogenin
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BMP3. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	BMP3
Synonyms	BMP3A
Function	Growth factor of the TGF-beta superfamily that plays an essential role in developmental process by inducing and patterning early skeletal formation and by negatively regulating bone density. Antagonizes the ability of certain osteogenic BMPs to induce osteoprogenitor differentiation and ossification (PubMed:11138004, PubMed:15269709). Initiates signaling cascades by associating with type II receptor ACVR2B to activate SMAD2-dependent and SMAD-independent signaling cascades including TAK1 and JNK pathways (PubMed:31665064).
Cellular Location	Secreted.
Tissue Location	Expressed in adult and fetal cartilage.