Anti-Carboxypeptidase N 1 Antibody

Western blot analysis of Carboxypeptidase N 1 expression in mouse liver (A), rat liver (B) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 48 kD)
Immunohistochemical analysis of Carboxypeptidase N 1 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P15169
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	52286 Da

Additional Information
Gene ID	1369
Other Names	ACBP; Carboxypeptidase N catalytic chain; CPN; Anaphylatoxin inactivator; Arginine carboxypeptidase; Carboxypeptidase N polypeptide 1; Carboxypeptidase N small subunit; Kininase-1; Lysine carboxypeptidase; Plasma carboxypeptidase B; Serum carboxypeptidase N; SCPN
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Carboxypeptidase N 1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CPN1
Synonyms	ACBP
Function	Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.
Cellular Location	Secreted, extracellular space.
Tissue Location	Synthesized in the liver and secreted in plasma.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.