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>   首页   >   产品   >   一抗   >   其他   >   Anti-CstF-64 Antibody   

Anti-CstF-64 Antibody

Rabbit polyclonal antibody to CstF-64

     
  • 1 - Anti-CstF-64 Antibody AP59527
    Western blot analysis of CstF-64 expression in mouse spleen (A), rat spleen (B), rat brain (C) whole cell lysates.
  • 2 - Anti-CstF-64 Antibody AP59527
    Immunohistochemical analysis of CstF-64 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • 3 - Anti-CstF-64 Antibody AP59527
    Immunofluorescent analysis of CstF-64 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IP, IF/IC, IHC
Primary Accession P33240
Other Accession Q8BIQ5
Reactivity Human, Mouse, Rat, Zebrafish, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 60959 Da
Additional Information
Gene ID 1478
Other Names Cleavage stimulation factor subunit 2; CF-1 64 kDa subunit; Cleavage stimulation factor 64 kDa subunit; CSTF 64 kDa subunit; CstF-64
Target/Specificity KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CstF-64. The exact sequence is proprietary.
Dilution WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
IP~~N/A
IF/IC~~N/A
IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name CSTF2
Function One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs.
Cellular Location Nucleus. Note=Localized with DDX1 in cleavage bodies.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CstF-64. The exact sequence is proprietary.

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