Anti-FGF5 Antibody

Western blot analysis of FGF5 expression in mouse liver (A), rat liver (B) whole cell lysates. (Predicted band size: 29 kD; Observed band size: 30 kD)
Immunohistochemical analysis of FGF5 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P12034
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	29551 Da

Additional Information
Gene ID	2250
Other Names	Fibroblast growth factor 5; FGF-5; Heparin-binding growth factor 5; HBGF-5; Smag-82
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human FGF5. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	FGF5
Function	Plays an important role in the regulation of cell proliferation and cell differentiation. Required for normal regulation of the hair growth cycle. Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase (By similarity).
Cellular Location	Secreted.
Tissue Location	Expressed in neonatal brain.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.