Anti-Glypican 5 Antibody

Western blot analysis of Glypican 5 expression in Hela (A), mouse brain (B), mouse lung (C), mouse liver (D), rat brain (E), rat lung (F) whole cell lysates. (Predicted band size: 63 kD; Observed band size: 70 kD)
Immunofluorescent analysis of Glypican 5 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P78333
Reactivity	Human, Mouse, Rat, Bovine, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	63707 Da

Additional Information
Gene ID	2262
Other Names	Glypican-5
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Glypican 5. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GPC5
Function	Cell surface proteoglycan that bears heparan sulfate.
Cellular Location	Cell membrane; Lipid-anchor, GPI- anchor; Extracellular side
Tissue Location	In adult, primarily expressed in the brain. Also detected in fetal brain, lung and liver.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.