Anti-GYS1 Antibody [KO/KD Validated]

Western blot analysis of GYS1 expression in HEK293T (A), LO2 (B), HepG2 (C), AML12 (D), H9C2 (E) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 95 kD)
Immunohistochemical analysis of GYS1 staining in human lymph formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of GYS1 expression in wild type (WT) and knockdown (KD) HeLa cell lysates.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P13807
Reactivity	Human, Mouse, Rat, Rabbit, Pig, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	83786 Da

Additional Information
Gene ID	2997
Other Names	GYS; Glycogen [starch] synthase, muscle
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GYS1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GYS1 (HGNC:4706)
Synonyms	GYS
Function	Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by adding new glucose units to it. In this context, glycogen synthase transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
Tissue Location	Expressed in skeletal muscle and most other cell types where glycogen is present.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.