Anti-Gamma-catenin Antibody

Rabbit polyclonal antibody to Gamma-catenin

Western blot analysis of Gamma-catenin expression in HEK293T (A), Hela (B), H1688 (C), mouse liver (D), rat liver (E) whole cell lysates.
Immunohistochemical analysis of Gamma-catenin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Gamma-catenin staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC, IHC
Primary Accession	P14923
Other Accession	Q02257
Reactivity	Human, Mouse, Rat, Rabbit
Host	Rabbit
Clonality	Polyclonal
Calculated MW	81745 Da

Additional Information
Gene ID	3728
Other Names	CTNNG; DP3; Junction plakoglobin; Catenin gamma; Desmoplakin III; Desmoplakin-3
Target/Specificity	Recognizes endogenous levels of Gamma-catenin protein.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF/IC (1/100 - 1/500) IF/IC~~N/A IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF/IC (1/100 - 1/500)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	JUP (HGNC:6207)
Function	Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E- cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity).
Cellular Location	Cell junction, adherens junction. Cell junction, desmosome. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein. Cytoplasm {ECO:0000250\|UniProtKB:Q9PVF7}. Cell junction {ECO:0000250\|UniProtKB:Q9PVF7}. Nucleus {ECO:0000250\|UniProtKB:Q9PVF7} Note=Cytoplasmic in a soluble and membrane-associated form. Colocalizes with DSG4 at desmosomes (PubMed:21495994)
Tissue Location	Expressed in the heart (at protein level).

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.