Anti-LMX1B Antibody

Rabbit polyclonal antibody to LMX1B

Western blot analysis of LMX1B expression in Hela (A), H446 (B) whole cell lysates.
Immunohistochemical analysis of LMX1B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IP, IHC
Primary Accession	O60663
Reactivity	Human, Pig, Bovine, Drosophila
Host	Rabbit
Clonality	Polyclonal
Calculated MW	44917 Da

Additional Information
Gene ID	4010
Other Names	LIM homeobox transcription factor 1-beta; LIM/homeobox protein 1.2; LMX-1.2; LIM/homeobox protein LMX1B
Target/Specificity	Recognizes endogenous levels of LMX1B protein.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IP (1/10 - 1/100) IP~~N/A IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IP (1/10 - 1/100)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	LMX1B
Function	Transcription factor involved in the regulation of podocyte- expressed genes (PubMed:24042019, PubMed:28059119). Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels.
Cellular Location	Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00108}.
Tissue Location	Expressed in most tissues. Highest levels in testis, thyroid, duodenum, skeletal muscle, and pancreatic islets

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.