Anti-EP4 Antibody

Western blot analysis of EP4 expression in HEK293T (A), Hela (B), H1688 (C), mouse lung (D), mouse kidney (E) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 68 kD)
Immunofluorescent analysis of EP4 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P35408
Reactivity	Human, Mouse, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	53119 Da

Additional Information
Gene ID	5734
Other Names	PTGER2; Prostaglandin E2 receptor EP4 subtype; PGE receptor EP4 subtype; PGE2 receptor EP4 subtype; Prostanoid EP4 receptor
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EP4. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	PTGER4
Synonyms	PTGER2
Function	Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
Cellular Location	Cell membrane; Multi-pass membrane protein.
Tissue Location	High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.