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Anti-VDR Antibody

Rabbit polyclonal antibody to VDR

     
  • 1 - Anti-VDR Antibody AP59729
    Western blot analysis of VDR expression in HCT116 (A), HEK293T (B), A549 (C), CT26 (D), PC12 (E) whole cell lysates.
  • 2 - Anti-VDR Antibody AP59729
    Immunohistochemical analysis of VDR staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • 3 - Anti-VDR Antibody AP59729
    Immunofluorescent analysis of VDR staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF/IC, IHC
Primary Accession P11473
Other Accession P48281
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 48289 Da
Additional Information
Gene ID 7421
Other Names NR1I1; Vitamin D3 receptor; VDR; 1, 25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1
Target/Specificity Recognizes endogenous levels of VDR protein.
Dilution WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF/IC (1/100 - 1/500)
IF/IC~~N/A
IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name VDR (HGNC:12679)
Synonyms NR1I1
Function Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells (PubMed:10678179, PubMed:15728261, PubMed:16913708, PubMed:28698609, PubMed:37478846). Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR (PubMed:28698609). The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes (PubMed:28698609). Plays a central role in calcium homeostasis (By similarity). Also functions as a receptor for the secondary bile acid lithocholic acid (LCA) and its metabolites (PubMed:12016314, PubMed:32354638).
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00407, ECO:0000269|PubMed:12145331, ECO:0000269|PubMed:16207705, ECO:0000269|PubMed:28698609}. Cytoplasm Note=Localizes mainly to the nucleus (PubMed:12145331, PubMed:28698609). Translocated into the nucleus via both ligand- dependent and ligand-independent pathways; ligand-independent nuclear translocation is mediated by IPO4 (PubMed:16207705)
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human VDR. The exact sequence is proprietary.

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