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Anti-14-3-3 epsilon Antibody

Rabbit polyclonal antibody to 14-3-3 epsilon

     
  • 1 - Anti-14-3-3 epsilon Antibody AP59733
    Western blot analysis of 14-3-3 epsilon expression in HEK293T (A), Hela (B), H1688 (C), mouse liver (D), mouse testis (E), rat liver (F), rat testis (G) whole cell lysates.
  • 3 - Anti-14-3-3 epsilon Antibody AP59733
    Immunofluorescent analysis of 14-3-3 epsilon staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF/IC
Primary Accession P62258
Other Accession P62259
Reactivity Human, Mouse, Rat, Zebrafish, Monkey, Chicken, Bovine, SARS
Host Rabbit
Clonality Polyclonal
Calculated MW 29174 Da
Additional Information
Gene ID 7531
Other Names 14-3-3 protein epsilon; 14-3-3E
Target/Specificity Recognizes endogenous levels of 14-3-3 epsilon protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
IF/IC~~N/A
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name YWHAE
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:21189250). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:35343654). Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326). Plays a positive role in the antiviral signaling pathway upstream of TBK1 via interaction with RIGI (PubMed:37555661). Mechanistically, directs RIGI redistribution from the cytosol to mitochondrial associated membranes where it mediates MAVS-dependent innate immune signaling during viral infection (PubMed:22607805). Plays a role in proliferation inhibition and cell cycle arrest by exporting HNRNPC from the nucleus to the cytoplasm to be degraded by ubiquitination (PubMed:37599448).
Cellular Location Nucleus. Cytoplasm Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human 14-3-3 epsilon. The exact sequence is proprietary.

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