Anti-CNT2 Antibody

Rabbit polyclonal antibody to CNT2

Western blot analysis of CNT2 expression in HEK293T (A) whole cell lysates.
Immunohistochemical analysis of CNT2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	O43868
Other Accession	O88627
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	71926 Da

Additional Information
Gene ID	9153
Other Names	CNT2; Sodium/nucleoside cotransporter 2; Concentrative nucleoside transporter 2; CNT 2; hCNT2; Na(+)/nucleoside cotransporter 2; Sodium-coupled nucleoside transporter 2; Sodium/purine nucleoside co-transporter; SPNT; Solute carrier family 28 member 2
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CNT2. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	SLC28A2
Synonyms	CNT2
Function	Sodium-dependent and purine-selective transporter (PubMed:10087507, PubMed:9435697). Exhibits the transport characteristics of the nucleoside transport system cif or N1 subtype (N1/cif) (selective for purine nucleosides and uridine) (PubMed:10087507, PubMed:21795683, PubMed:9435697). Plays a critical role in specific uptake and salvage of purine nucleosides in kidney and other tissues (PubMed:9435697). May contribute to regulate the transport of organic compounds in testes across the blood-testis- barrier (Probable).
Cellular Location	Membrane; Multi-pass membrane protein. Apicolateral cell membrane; Multi-pass membrane protein. Note=Localized to the apicolateral membranes of Sertoli cells and vascular endothelial cells in testis
Tissue Location	Expressed in heart and skeletal muscle followed by liver, kidney, intestine, pancreas, placenta and brain (PubMed:9435697). Weak expression in lung (PubMed:9435697). In testis, primarily localized to the apicolateral membranes of Sertoli cells and vascular endothelial cells, and weakly expressed in Leydig cells, peritubular myoid cells and germ cells (PubMed:35307651)