Anti-HORMAD1 Antibody

Western blot analysis of HORMAD1 expression in mouse kidney (A) whole cell lysates. (Predicted band size: 45 kD; Observed band size: 45 kD)
Immunofluorescent analysis of HORMAD1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q86X24
Reactivity	Human, Mouse, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	45200 Da

Additional Information
Gene ID	84072
Other Names	NOHMA; HORMA domain-containing protein 1; Cancer/testis antigen 46; CT46; Newborn ovary HORMA protein
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human HORMAD1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	HORMAD1 (HGNC:25245)
Function	Plays a key role in meiotic progression. Regulates 3 different functions during meiosis: ensures that sufficient numbers of processed DNA double-strand breaks (DSBs) are available for successful homology search by increasing the steady-state numbers of single- stranded DSB ends. Promotes synaptonemal-complex formation independently of its role in homology search. Plays a key role in the male mid-pachytene checkpoint and the female meiotic prophase checkpoint: required for efficient build-up of ATR activity on unsynapsed chromosome regions, a process believed to form the basis of meiotic silencing of unsynapsed chromatin (MSUC) and meiotic prophase quality control in both sexes.
Cellular Location	Nucleus {ECO:0000250\|UniProtKB:Q9D5T7}. Chromosome {ECO:0000250\|UniProtKB:Q9D5T7}. Note=Preferentially localizes to unsynapsed or desynapsed chromosomal regions during the prophase I stage of meiosis. TRIP13 is required for depletion from synapsed chromosomes. The expression of the phosphorylated form at Ser- 377 is restricted to unsynapsed chromosomal regions (By similarity) {ECO:0000250\|UniProtKB:Q9D5T7}
Tissue Location	Testis-specific. Over-expressed in carcinomas.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.