Anti-APOA1BP Antibody

Rabbit polyclonal antibody to APOA1BP

Western blot analysis of APOA1BP expression in DLD (A), mouse liver (B), mouse heart (C), rat kidney (D), rat heart (E) whole cell lysates.
Immunofluorescent analysis of APOA1BP staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q8NCW5
Other Accession	Q8K4Z3
Reactivity	Human, Mouse, Rat, Pig, Bovine, Drosophila
Host	Rabbit
Clonality	Polyclonal
Calculated MW	31675 Da

Additional Information
Gene ID	128240
Other Names	AIBP; YJEFN1; NAD(P)H-hydrate epimerase; Apolipoprotein A-I-binding protein; AI-BP; NAD(P)HX epimerase; YjeF N-terminal domain-containing protein 1; YjeF_N1
Target/Specificity	Recognizes endogenous levels of APOA1BP protein.
Dilution	WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	NAXE (HGNC:18453)
Function	Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity) (PubMed:27616477). This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX (By similarity). Accelerates cholesterol efflux from endothelial cells to high-density lipoprotein (HDL) and thereby regulates angiogenesis (PubMed:23719382).
Cellular Location	Mitochondrion {ECO:0000255\|HAMAP-Rule:MF_03159}. Secreted {ECO:0000255\|HAMAP-Rule:MF_03159, ECO:0000269\|PubMed:11991719}. Note=In sperm, secretion gradually increases during capacitation. {ECO:0000255\|HAMAP-Rule:MF_03159}
Tissue Location	Ubiquitously expressed, with highest levels in kidney, heart and liver. Present in cerebrospinal fluid and urine but not in serum from healthy patients. Present in serum of sepsis patients (at protein level).

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.