Anti-CD200R Antibody

Western blot analysis of CD200R expression in AML12 (A), PC12 (B), HEK293T (C), A549 (D), LO2 (E) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 36; 75 kD)
Immunofluorescent analysis of CD200R staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q8TD46
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	39041 Da

Additional Information
Gene ID	131450
Other Names	CD200R; CRTR2; MOX2R; OX2R; Cell surface glycoprotein CD200 receptor 1; CD200 cell surface glycoprotein receptor; Cell surface glycoprotein OX2 receptor 1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD200R. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CD200R1
Synonyms	CD200R, CRTR2, MOX2R, OX2R
Function	Inhibitory receptor for the CD200/OX2 cell surface glycoprotein. Limits inflammation by inhibiting the expression of pro- inflammatory molecules including TNF, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.
Cellular Location	[Isoform 1]: Cell membrane; Single-pass type I membrane protein [Isoform 2]: Secreted.
Tissue Location	Expressed in granulocytes, monocytes, most T-cells, neutrophils, basophils and a subset of NK, NKT and B-cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.