TRAM2 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

TRAM2 Antibody (C-term) (Cat. #AP5996b) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TRAM2 antibody detected the TRAM2 protein (arrow).
TRAM2 antibody (C-term) (Cat. #AP5996b) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRAM2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q15035
Other Accession	NP_036420.1
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	43328 Da
Antigen Region	342-370 aa

Additional Information
Gene ID	9697
Other Names	Translocating chain-associated membrane protein 2, TRAM2, KIAA0057
Target/Specificity	This TRAM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-370 amino acids from the C-terminal region of human TRAM2.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	TRAM2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	TRAM2
Synonyms	KIAA0057
Function	Necessary for collagen type I synthesis. May couple the activity of the ER Ca(2+) pump SERCA2B with the activity of the translocon. This coupling may increase the local Ca(2+) concentration at the site of collagen synthesis, and a high Ca(2+) concentration may be necessary for the function of molecular chaperones involved in collagen folding. Required for proper insertion of the first transmembrane helix N-terminus of TM4SF20 into the ER lumen, may act as a ceramide sensor for regulated alternative translocation (RAT) (PubMed:27499293).
Cellular Location	Membrane; Multi-pass membrane protein

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.