Anti-p38 (Phospho-T180) Antibody

Western blot analysis of p38 (Phospho-T180) expression in zebrafish (A) whole cell lysates. (Predicted band size: 41 kD; Observed band size: 43 kD)
Immunofluorescent analysis of p38 (Phospho-T180) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blot analysis of p38 (Phospho-T180) expression in mouse liver (A), rat liver (B) whole cell lysates.
Direct ELISA antibody dose-response curve using Anti-p38 (Phospho-T180) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
Western blot analysis of p38 (pT180) expression in mouse liver (A), rat liver (B) whole cell lysates.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q16539
Reactivity	Human, Mouse, Rat, Zebrafish, Dog
Host	Rabbit
Clonality	Polyclonal

Additional Information
Other Names	CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; Mitogen-activated protein kinase 14; MAP kinase 14; MAPK 14; Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; CSBP; MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha; MAP kinase p38 alpha; Stress-activated protein kinase 2a; SAPK2a
Target/Specificity	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T180 of human p38 protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt