Anti-CSK Antibody

Western blot analysis of CSK expression in mouse spleen (A), rat spleen (B) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 50 kD)
Immunofluorescent analysis of CSK staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P41240
Reactivity	Human, Mouse, Rat, Bovine, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	50704 Da

Additional Information
Gene ID	1445
Other Names	Tyrosine-protein kinase CSK; C-Src kinase; Protein-tyrosine kinase CYL
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CSK. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CSK
Function	Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C- terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN, CSK or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK. May act as a negative regulator of EGFR and STAT3 signaling pathways (PubMed:26918609).
Cellular Location	Cytoplasm {ECO:0000250\|UniProtKB:P41241}. Cell membrane {ECO:0000250\|UniProtKB:P41241}. Note=Mainly cytoplasmic, also present in lipid rafts. {ECO:0000250\|UniProtKB:P41241}
Tissue Location	Expressed in lung and macrophages.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.