Anti-MKK2 (pT394) Antibody

Rabbit polyclonal antibody to MKK2 (pT394)

Western blot analysis of MKK2 (pT394) expression in HEK293T (A), Hela (B), DLD (C) whole cell lysates.
Immunohistochemical analysis of MKK2 (pT394) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IP, IHC
Primary Accession	P36507
Reactivity	Human, Monkey
Host	Rabbit
Clonality	Polyclonal
Calculated MW	44424 Da

Additional Information
Gene ID	5605
Other Names	MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein kinase kinase 2; MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2
Target/Specificity	Recognizes endogenous levels of MKK2 (pT394) protein.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IP (1/10 - 1/100) IP~~N/A IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IP (1/10 - 1/100)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	MAP2K2
Synonyms	MEK2, MKK2, PRKMK2
Function	Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed:29433126).
Cellular Location	Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane localization is probably regulated by its interaction with KSR1.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.