Anti-Paxillin (Phospho-Y118) Antibody

Western blot analysis of Paxillin (Phospho-Y118) expression in mouse lung (A), mouse heart (B) whole cell lysates. (Predicted band size: 64 kD; Observed band size: 68 kD)
Immunofluorescent analysis of Paxillin (Phospho-Y118) staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P49023
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	64505 Da

Additional Information
Gene ID	5829
Other Names	Paxillin
Target/Specificity	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y118 of human Paxillin protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	PXN (HGNC:9718)
Function	Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion.
Cellular Location	Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex {ECO:0000250\|UniProtKB:Q8VI36}. Note=Colocalizes with integrins at the cell periphery. Colocalize with PXN to membrane ruffles and the leading edge of migrating cells (PubMed:23128389). {ECO:0000250, ECO:0000269\|PubMed:23128389}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.