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Anti-CLIC4 Antibody [KO/KD Validated]

Western blot analysis of CLIC4 expression in COS7 (A), LOVO (B), CT26 (C), PC12 (D) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 29 kD)
Immunohistochemical analysis of CLIC4 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of CLIC4 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of CLIC4 expression in wild type (WT) and knockdown (KD) HeLa cell lysates.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q9Y696
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	28772 Da

Additional Information
Gene ID	25932
Other Names	Chloride intracellular channel protein 4; Intracellular chloride ion channel protein p64H1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CLIC4. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CLIC4 {ECO:0000303\|PubMed:12163372, ECO:0000312\|HGNC:HGNC:13518}
Function	In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor (PubMed:25581026, PubMed:37759794). Can insert into membranes and form voltage-dependent multi-ion conductive channels. Membrane insertion seems to be redox-regulated and may occur only under oxidizing conditions (By similarity) (PubMed:16176272). Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Promotes cell-surface expression of HRH3.
Cellular Location	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic vesicle membrane; Single-pass membrane protein. Nucleus. Cell membrane; Single-pass membrane protein. Mitochondrion {ECO:0000250\|UniProtKB:Q9Z0W7}. Cell junction. Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:Q9Z0W7}; Single-pass membrane protein {ECO:0000250\|UniProtKB:Q9Z0W7}. Note=Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles. Might not be present in the nucleus of cardiac cells. {ECO:0000250\|UniProtKB:Q9Z0W7, ECO:0000269\|PubMed:14569596}
Tissue Location	Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol