Anti-HECW2 Antibody

Rabbit polyclonal antibody to HECW2

Western blot analysis of HECW2 expression in H1792 (A), A549 (B), PMVEC (C), AML12 (D) whole cell lysates.
Immunofluorescent analysis of HECW2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q9P2P5
Other Accession	Q6I6G8
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	175769 Da

Additional Information
Gene ID	57520
Other Names	KIAA1301; NEDL2; E3 ubiquitin-protein ligase HECW2; HECT, C2 and WW domain-containing protein 2; NEDD4-like E3 ubiquitin-protein ligase 2
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HECW2. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	HECW2
Synonyms	KIAA1301, NEDL2
Function	E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73 (PubMed:12890487). Involved in the regulation of mitotic metaphase/anaphase transition (PubMed:24163370).
Cellular Location	Cytoplasm. Cytoplasm, cytoskeleton, spindle
Tissue Location	Predominantly expressed in adult brain, lung and heart.