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Anti-Alpha-2A Adrenergic Receptor Antibody

Rabbit polyclonal antibody to Alpha-2A Adrenergic Receptor

Western blot analysis of Alpha-2A Adrenergic Receptor expression in Hela (A), A2780 (B) whole cell lysates.
Immunofluorescent analysis of Alpha-2A Adrenergic Receptor staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IP, IF/IC
Primary Accession	P08913
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	50647 Da

Additional Information
Gene ID	150
Other Names	ADRA2R; ADRAR; Alpha-2A adrenergic receptor; Alpha-2 adrenergic receptor subtype C10; Alpha-2A adrenoreceptor; Alpha-2A adrenoceptor; Alpha-2AAR
Target/Specificity	Recognizes endogenous levels of Alpha-2A Adrenergic Receptor protein.
Dilution	WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100) IP~~N/A IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	ADRA2A (HGNC:281)
Synonyms	ADRA2R, ADRAR
Function	Alpha-2 adrenergic receptors mediate the catecholamine- induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p- octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol.
Cellular Location	Cell membrane; Multi-pass membrane protein

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Alpha-2A Adrenergic Receptor. The exact sequence is proprietary.

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¥ 1,500.00

Cat# AP60214

Size:

50ul

100 µl

Price:

¥ 1,500.00

¥ 2,600.00

Quantity:

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Availability: 3-5 days

询价

Anti-Alpha-2A Adrenergic Receptor Antibody

Rabbit polyclonal antibody to Alpha-2A Adrenergic Receptor

BACKGROUND

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