Anti-CDK2AP1 Antibody

Western blot analysis of CDK2AP1 expression in HEK293T (A), HEK293T overexpression (B), HEK293T mutant (C), HEK293T knockout (D) whole cell lysates. (Predicted band size: 12 kD; Observed band size: 12 kD)
Immunohistochemical analysis of CDK2AP1 staining in human apex of heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	O14519
Reactivity	Human, Mouse, Rat, Chicken, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	12365 Da

Additional Information
Gene ID	8099
Other Names	CDKAP1; DOC1; Cyclin-dependent kinase 2-associated protein 1; CDK2-associated protein 1; Deleted in oral cancer 1; DOC-1; Putative oral cancer suppressor
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDK2AP1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CDK2AP1
Synonyms	CDKAP1, DOC1
Function	Inhibitor of cyclin-dependent kinase CDK2 (By similarity). Also acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:20523938, PubMed:28977666).
Cellular Location	Nucleus. Chromosome

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.