Anti-GBA3 Antibody

Western blot analysis of GBA3 expression in LO2 (A), HepG2 (B), CT26 (C), rat liver (D) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 54 kD)
Immunofluorescent analysis of GBA3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q9H227
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	53696 Da

Additional Information
Gene ID	57733
Other Names	CBG; CBGL1; Cytosolic beta-glucosidase; Cytosolic beta-glucosidase-like protein 1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GBA3. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GBA3 (HGNC:19069)
Synonyms	CBG, CBGL1
Function	Neutral cytosolic beta-glycosidase with a broad substrate specificity that could play a role in the catabolism of glycosylceramides (PubMed:11389701, PubMed:11784319, PubMed:17595169, PubMed:20728381, PubMed:26724485, PubMed:33361282). Has a significant glucosylceramidase activity in vitro (PubMed:17595169, PubMed:26724485). However, that activity is relatively low and its significance in vivo is not clear (PubMed:17595169, PubMed:20728381, PubMed:26724485). Hydrolyzes galactosylceramides/GalCers, glucosylsphingosines/GlcSphs and galactosylsphingosines/GalSphs (PubMed:17595169). However, the in vivo relevance of these activities is unclear (PubMed:17595169). It can also hydrolyze a broad variety of dietary glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens in vitro and could therefore play a role in the metabolism of xenobiotics (PubMed:11784319). Possesses transxylosylase activity in vitro using xylosylated ceramides/XylCers (such as beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine) as xylosyl donors and cholesterol as acceptor (PubMed:33361282). Could also play a role in the catabolism of cytosolic sialyl free N-glycans (PubMed:26193330).
Cellular Location	Cytoplasm, cytosol
Tissue Location	Present in small intestine (at protein level). Expressed in liver, small intestine, colon, spleen and kidney. Down- regulated in renal cell carcinomas and hepatocellular carcinomas