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Anti-GNAT1 Antibody

Western blot analysis of GNAT1 expression in mouse eyes (A), rat eyes (B) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 40 kD)
Immunohistochemical analysis of GNAT1 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of GNAT1 staining in 4T1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of GNAT1 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P11488
Reactivity	Human, Mouse, Rat, Bovine, Dog
Host	Rabbit
Clonality	Polyclonal
Calculated MW	40041 Da

Additional Information
Gene ID	2779
Other Names	GNATR; Guanine nucleotide-binding protein G(t) subunit alpha-1; Transducin alpha-1 chain
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GNAT1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GNAT1 (HGNC:4393)
Synonyms	GNATR
Function	Alpha subunit of the heterotrimeric guanine nucleotide- binding protein (G protein) that transduces signals from the G protein- coupled photoreceptor rhodopsin (RHO). Required for normal RHO-mediated light perception in retinal rod photoreceptors (PubMed:22190596). Functions as a GTPase that contains the guanine nucleotide binding site and cycles between an active GTP-bound state and an inactive GDP-bound state. Under dim light conditions, RHO promotes GDP release and GTP binding. Activates downstream effectors, including cone cGMP- phosphodiesterase (PDE6), to mediate the phototransduction cascade (By similarity). Stimulates PDE6 by binding and repositioning the PDE6 gamma inhibitory subunits (PDE6G) to promote catalytic activity (By similarity).
Cellular Location	Photoreceptor outer segment membrane {ECO:0000250\|UniProtKB:P04695}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P04695}. Membrane {ECO:0000250\|UniProtKB:P04695}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P04695}. Photoreceptor inner segment {ECO:0000250\|UniProtKB:P20612}. Note=Localizes mainly in the outer segment in the dark-adapted state, whereas is translocated to the inner part of the photoreceptors in the light-adapted state. During dark- adapted conditions, in the presence of UNC119 mislocalizes from the outer segment to the inner part of rod photoreceptors which leads to decreased photoreceptor damage caused by light {ECO:0000250\|UniProtKB:P20612}
Tissue Location	Rod photoreceptor cells (PubMed:1614872). Predominantly expressed in the retina followed by the ciliary body, iris and retinal pigment epithelium (PubMed:22190596)