Anti-NF-kappaB p65 (pT254) Antibody

Rabbit polyclonal antibody to NF-kappaB p65 (pT254)

Western blot analysis of NF-kappaB p65 (pT254) expression in MCF7-LPS-30mins (A), MCF7-LPS-15mins (B), MCF7-LPS-5mins (C), MCF7 (D), Hela-TNFa-15mins (E), Hela-TNFa-5mins (F), Hela (G) whole cell lysates.
Immunohistochemical analysis of NF-kappaB p65 (pT254) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Direct ELISA antibody dose-response curve using Anti-NF-kappaB p65 (pT254) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IP, IHC
Primary Accession	Q04206
Other Accession	Q04207
Reactivity	Human, Mouse, Rat, Monkey, Pig, Bovine
Host	Rabbit
Clonality	Polyclonal

Additional Information
Other Names	NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NF-kappaB p65 (pT254). The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IP (1/10 - 1/100) IP~~N/A IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IP (1/10 - 1/100)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt