Anti-TACSTD2 Antibody

Rabbit polyclonal antibody to TACSTD2

Western blot analysis of TACSTD2 expression in HEK293T (A), Hela (B), H1688 (C), mouse kidney (D), mouse lung (E), rat kidney (F), rat lung (G) whole cell lysates.
Immunohistochemical analysis of TACSTD2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of TACSTD2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC, IHC
Primary Accession	P09758
Other Accession	Q8BGV3
Reactivity	Human, Mouse, Rat, Pig
Host	Rabbit
Clonality	Polyclonal
Calculated MW	35709 Da

Additional Information
Gene ID	4070
Other Names	GA733-1; M1S1; TROP2; Tumor-associated calcium signal transducer 2; Cell surface glycoprotein Trop-2; Membrane component chromosome 1 surface marker 1; Pancreatic carcinoma marker protein GA733-1
Target/Specificity	Recognizes endogenous levels of TACSTD2 protein.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF/IC (1/100 - 1/500) IF/IC~~N/A IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF/IC (1/100 - 1/500)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	TACSTD2
Synonyms	GA733-1, M1S1, TROP2
Function	May function as a growth factor receptor.
Cellular Location	Membrane; Single-pass type I membrane protein.
Tissue Location	Placenta, pancreatic carcinoma cell lines.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.