Anti-WASF3 Antibody

Western blot analysis of WASF3 expression in mouse kidney (A), rat kidney (B) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 55 kD)
Immunohistochemical analysis of WASF3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of WASF3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q9UPY6
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	55293 Da

Additional Information
Gene ID	10810
Other Names	KIAA0900; SCAR3; WAVE3; Wiskott-Aldrich syndrome protein family member 3; WASP family protein member 3; Protein WAVE-3; Verprolin homology domain-containing protein 3
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human WASF3. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	WASF3
Synonyms	KIAA0900, SCAR3, WAVE3
Function	Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Required for regulation of cell morphology and cytoskeletal organization (PubMed:21834987).
Cellular Location	Cytoplasm, cytoskeleton.
Tissue Location	Expressed in ovary and brain.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.