Anti-EIF3K Antibody

Western blot analysis of EIF3K expression in MCF7 (A), C6 (B), mouse testis (C), HEK293T (D) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 30; 25 kD)
Immunohistochemical analysis of EIF3K staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q9UBQ5
Reactivity	Human, Mouse, Rat, Rabbit, Pig, Bovine, Dog, Sheep, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	25060 Da

Additional Information
Gene ID	27335
Other Names	EIF3S12; Eukaryotic translation initiation factor 3 subunit K; eIF3k; Eukaryotic translation initiation factor 3 subunit 12; Muscle-specific gene M9 protein; PLAC-24; eIF-3 p25; eIF-3 p28
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EIF3K. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	EIF3K {ECO:0000255\|HAMAP-Rule:MF_03010}
Function	Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl- tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).
Cellular Location	Nucleus {ECO:0000255\|HAMAP-Rule:MF_03010, ECO:0000269\|PubMed:15327989}. Cytoplasm {ECO:0000255\|HAMAP- Rule:MF_03010, ECO:0000269\|PubMed:15327989}
Tissue Location	Ubiquitous, with the highest levels of expression in brain, testis and kidney.