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>   首页   >   产品   >   一抗   >   其他   >   Anti-4EBP1 (pT69) Antibody   

Anti-4EBP1 (pT69) Antibody

Rabbit polyclonal antibody to 4EBP1 (pT69)

     
  • 1 - Anti-4EBP1 (pT69) Antibody AP60456
    Western blot analysis of 4EBP1 (pT69) expression in U2OS (A), DLD (B) whole cell lysates.
  • 2 - Anti-4EBP1 (pT69) Antibody AP60456
    Immunohistochemical analysis of 4EBP1 (pT69) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession Q13541
Other Accession Q60876
Reactivity Human, Mouse, Rat, Monkey
Host Rabbit
Clonality Polyclonal
Calculated MW 12580 Da
Additional Information
Gene ID 1978
Other Names EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Target/Specificity Recognizes endogenous levels of 4EBP1 (pT69) protein.
Dilution WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name EIF4EBP1
Function Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Cellular Location Cytoplasm. Nucleus. Note=Localization to the nucleus is unaffected by phosphorylation status. {ECO:0000250|UniProtKB:Q60876}
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human 4EBP1 (pT69). The exact sequence is proprietary.

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