Anti-Adenosine A3 Receptor Antibody

Rabbit polyclonal antibody to Adenosine A3 Receptor

Western blot analysis of Adenosine A3 Receptor expression in MCF7 (A), H1792 (B) whole cell lysates.
Immunofluorescent analysis of Adenosine A3 Receptor staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P0DMS8
Other Accession	Q61618
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	36185 Da

Additional Information
Gene ID	140
Other Names	Adenosine receptor A3
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Adenosine A3 Receptor. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	ADORA3 (HGNC:268)
Function	[Isoform 2]: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase (PubMed:8234299).
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:Q28309}; Multi-pass membrane protein
Tissue Location	Expressed in the lung and bone. Expressed at lower levels in osteosarcoma tissues (at protein level)

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.