Anti-A-RAF Antibody

Western blot analysis of A-RAF expression in MCF7 (A) whole cell lysates. (Predicted band size: 67 kD; Observed band size: 68 kD)
Immunohistochemical analysis of A-RAF staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P10398
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	67585 Da

Additional Information
Gene ID	369
Other Names	ARAF1; PKS; PKS2; Serine/threonine-protein kinase A-Raf; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human A-RAF. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ARAF
Synonyms	ARAF1, PKS, PKS2
Function	Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Phosphorylates PFKFB2 (PubMed:36402789).
Tissue Location	Predominantly in urogenital tissues.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.