Anti-KIF2C (Phospho-S95) Antibody

Western blot analysis of KIF2C (Phospho-S95) expression in H1688 (A), H446 (B) whole cell lysates. (Predicted band size: 81 kD; Observed band size: 81 kD)
Immunohistochemical analysis of KIF2C (Phospho-S95) Antibody staining in human carcinoma cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).
Immunofluorescent analysis of KIF2C (Phospho-S95) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	Q99661
Reactivity	Human, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	81313 Da

Additional Information
Gene ID	11004
Other Names	KNSL6; Kinesin-like protein KIF2C; Kinesin-like protein 6; Mitotic centromere-associated kinesin; MCAK
Target/Specificity	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S95 of human KIF2C protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	KIF2C
Synonyms	KNSL6
Function	In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (PubMed:21820309). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis (PubMed:19060894). Plays a role in chromosome congression and is required for the lateral to end- on conversion of the chromosome-microtubule attachment (PubMed:23891108).
Cellular Location	Cytoplasm, cytoskeleton. Nucleus {ECO:0000250\|UniProtKB:P70096} Chromosome, centromere. Chromosome, centromere, kinetochore. Note=Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Association with microtubule plus ends is also mediated by interaction with KIF18B. Centromeric localization requires the presence of BUB1 and SGO2. {ECO:0000250\|UniProtKB:P70096, ECO:0000269\|PubMed:17485487, ECO:0000269\|PubMed:21820309}
Tissue Location	Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis- specific.