Anti-SKIL Antibody

Rabbit polyclonal antibody to SKIL

Western blot analysis of SKIL expression in HEK293T (A), H446 (B), rat liver (C) whole cell lysates.
Immunohistochemical analysis of SKIL staining in human lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P12757
Other Accession	Q60665
Reactivity	Human, Mouse, Rat, Pig, Chicken, Bovine, Drosophila
Host	Rabbit
Clonality	Polyclonal
Calculated MW	76976 Da

Additional Information
Gene ID	6498
Other Names	SNO; Ski-like protein; Ski-related oncogene; Ski-related protein
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SKIL. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	SKIL
Synonyms	SNO
Function	May have regulatory role in cell division or differentiation in response to extracellular signals.
Tissue Location	Isoform SNON and isoform SNOA are widely expressed. Highest expression is found in skeletal muscle, followed by placenta and lung. Lowest expression in heart, brain and pancreas. Isoform SNOI expression is restricted to skeletal muscle

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.