Anti-L1CAM Antibody

Western blot analysis of L1CAM expression in HEK293T (A), A549 (B) whole cell lysates. (Predicted band size: 140 kD; Observed band size: 140 kD)
Immunohistochemical analysis of L1CAM staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of L1CAM staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P32004
Reactivity	Human, Mouse, Rat, Zebrafish
Host	Rabbit
Clonality	Polyclonal
Calculated MW	140003 Da

Additional Information
Gene ID	3897
Other Names	CAML1; MIC5; Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD171
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human L1CAM. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	L1CAM
Synonyms	CAML1, MIC5
Function	Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.
Cellular Location	Cell membrane; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:Q05695}. Cell projection, growth cone {ECO:0000250\|UniProtKB:Q05695}. Cell projection, axon. Cell projection, dendrite Note=Colocalized with SHTN1 in close apposition with actin filaments in filopodia and lamellipodia of axonalne growth cones of hippocampal neurons (By similarity). In neurons, detected predominantly in axons and cell body, weak localization to dendrites (PubMed:20621658) {ECO:0000250\|UniProtKB:Q05695, ECO:0000269\|PubMed:20621658}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.