Anti-L1CAM Antibody

Rabbit polyclonal antibody to L1CAM

Western blot analysis of L1CAM expression in HEK293T (A), A549 (B) whole cell lysates.
Immunofluorescent analysis of L1CAM staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P32004
Other Accession	P11627
Reactivity	Human, Mouse, Rat, Zebrafish
Host	Rabbit
Clonality	Polyclonal
Calculated MW	140003 Da

Additional Information
Gene ID	3897
Other Names	CAML1; MIC5; Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD171
Target/Specificity	Recognizes endogenous levels of L1CAM protein.
Dilution	WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	L1CAM
Synonyms	CAML1, MIC5
Function	Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.
Cellular Location	Cell membrane; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:Q05695}. Cell projection, growth cone {ECO:0000250\|UniProtKB:Q05695}. Cell projection, axon. Cell projection, dendrite Note=Colocalized with SHTN1 in close apposition with actin filaments in filopodia and lamellipodia of axonalne growth cones of hippocampal neurons (By similarity). In neurons, detected predominantly in axons and cell body, weak localization to dendrites (PubMed:20621658) {ECO:0000250\|UniProtKB:Q05695, ECO:0000269\|PubMed:20621658}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.