Anti-CD137 Antibody

Western blot analysis of CD137 expression in HEK293T (A), Hela (B), HepG2 (C), mouse brain (D), mouse spleen (E), rat spleen (F) whole cell lysates. (Predicted band size: 27 kD; Observed band size: 27 kD)
Immunofluorescent analysis of CD137 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q07011
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	27899 Da

Additional Information
Gene ID	3604
Other Names	CD137; ILA; Tumor necrosis factor receptor superfamily member 9; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA; CD137
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD137. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TNFRSF9
Synonyms	CD137, ILA
Function	Receptor for TNFSF9/4-1BBL. Conveys a signal that enhances CD8(+) T-cell survival, cytotoxicity, and mitochondrial activity, thereby promoting immunity against viruses and tumors (Probable).
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Expressed on the surface of activated T-cells.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.