Anti-DDX19B Antibody

Rabbit polyclonal antibody to DDX19B

Western blot analysis of DDX19B expression in LO2 (A), A375 (B), mouse kindey (C) whole cell lysates.
Immunohistochemical analysis of DDX19B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q9UMR2
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	53927 Da

Additional Information
Gene ID	11269
Other Names	DBP5; DDX19; TDBP; ATP-dependent RNA helicase DDX19B; DEAD box RNA helicase DEAD5; DEAD box protein 19B
Target/Specificity	Recognizes endogenous levels of DDX19B protein.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	DDX19B
Synonyms	DBP5, DDX19, TDBP
Function	ATP-dependent RNA helicase involved in mRNA export from the nucleus (PubMed:10428971). Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins (PubMed:10428971).
Cellular Location	Cytoplasm. Nucleus, nucleoplasm. Note=Associates with the nuclear pore complex cytoplasmic fibrils

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.