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Anti-Alpha-1D Adrenergic Receptor Antibody

Rabbit polyclonal antibody to Alpha-1D Adrenergic Receptor

Western blot analysis of Alpha-1D Adrenergic Receptor expression in H9C2 (A), Raw264.7 (B), U87MG (C) whole cell lysates.
Immunohistochemical analysis of Alpha-1D Adrenergic Receptor staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Alpha-1D Adrenergic Receptor staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC, IHC
Primary Accession	P25100
Other Accession	P97714
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	60463 Da

Additional Information
Gene ID	146
Other Names	ADRA1A; Alpha-1D adrenergic receptor; Alpha-1A adrenergic receptor; Alpha-1D adrenoreceptor; Alpha-1D adrenoceptor; Alpha-adrenergic receptor 1a
Target/Specificity	Recognizes endogenous levels of Alpha-1D Adrenergic Receptor protein.
Dilution	WB~~WB (1/500 - 1/2000), IHC (1/50 - 1/200), IF/IC (1/50 - 1/100) IF/IC~~N/A IHC~~WB (1/500 - 1/2000), IHC (1/50 - 1/200), IF/IC (1/50 - 1/100)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	ADRA1D
Synonyms	ADRA1A
Function	This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.
Cellular Location	Cell membrane; Multi-pass membrane protein.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Alpha-1D Adrenergic Receptor. The exact sequence is proprietary.

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¥ 1,500.00

Cat# AP60817

Size:

50ul

100 µl

Price:

¥ 1,500.00

¥ 2,600.00

Quantity:

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Availability: 3-5 days

询价

Anti-Alpha-1D Adrenergic Receptor Antibody

Rabbit polyclonal antibody to Alpha-1D Adrenergic Receptor

BACKGROUND

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